The cash-strapped operator confirmed to TTG consultations were already under way with a number of staff at its Peterborough base.
It comes just weeks after Cook confirmed it would further trim its UK retail network to 566 stores, placing more than 300 jobs at risk.
Cook will post its first-half results on Thursday (16 May), whereby it is expected to announce further details of its proposed cost-saving efforts.
Will Waggott, Cook’s chief of tour operating, said: “These proposals are part of our ongoing programme to drive greater efficiencies across the business, putting a rigorous focus on cost to ensure Thomas Cook is fit for the future.
“They have not been taken lightly and we are doing all we can to support our colleagues who are impacted through the consultation process.”
A spokesperson confirmed the move could mean up to 150 staff based in Peterborough leaving the business.