Rail union TSSA cancelled the industrial action after members voted to accept an improved pay offer.
The majority of members (80%) voted to accept the latest offer which the union said addresses their main concerns.
As a result, the walkout, which was due to begin on Saturday (27 May), will now not go ahead. TSSA had already suspended strike action planned for Wednesday (24 May).
TSSA interim organising director, Mel Taylor, said: "Elizabeth Line’s new offer recognises the uniquely multi-skilled nature of our members, who operate the world’s only fully digital railway.
"Our members were being paid thousands of pounds less than colleagues performing similar roles on other parts of the Transport for London (TfL) network."
