The budget carrier has put 60,000 seats on sale from Norwich across three twice-weekly routes to Alicante, Faro and Malta, which will take off in the spring.
Ryanair said the launch would create more choice for holidaymakers in Norfolk and lay down a challenge to "sky high" fares at the airport.
In addition, Ryanair said the new base would drive inbound tourism to the region and create more than 60 new jobs locally. Promotional fares lead in from £29.99pp.
"Our new Norwich operation will not only bring competitive fares and a wider selection of summer routes to our customers in Norwich, and the wider Norfolk region, but will support important regional development through increased connectivity, inbound tourism and local job creation," said Jade Kirwan, Ryanair head of communication.
"We look forward to further developing our partnership with Norwich airport."
Richard Pace, Norwich airport managing director, added: "This is fantastic news for passengers and the first time that Ryanair has flown from Norwich airport.
"Alicante, Faro and Malta are hugely popular destinations. and with twice-weekly flights. it means we can offer passengers even more great choice from their local airport. We’re delighted to be increasing connectivity for local communities and look forward to growing our partnership with Ryanair."
