The 17-day Sub-Antarctic Safari voyage will depart Chile’s capital Santiago on 4 November, wrapping up in Ushuaia, Argentina on 20 November.
Guests will be able to explore the Falkland Islands and South Georgia, tracing Sir Ernest Shackleton’s historic journey to the islands.
The ship is named after marine biologist, oceanographer and explorer Dr Sylvia Earle.
Hayley Peacock-Gower, chief marketing officer at Aurora Expeditions, said the ship would serve as a "floating ambassador" for the world’s conservation efforts, with its itineraries placing a focus on marine education and conservation.