Heathrow was inundated by complaints last month after it trialled new flight paths over areas of Berkshire and Surrey.
The airport received an average of 500 complaints a day – the highest in its history – according to The Sunday Times.
The airport has apologised for the disturbance caused by the trial.
Heathrow is one of a number of airports that have designed new flight paths. The changes are part of a plan to revamp the UK’s airspace by 2020.
A petition launched by residents in Ascot has attracted more than 5,600 signatures.
“The noise has increased massively since early July,” said organiser Amanda Smerczak.