Flights will depart from Leeds Bradford, Manchester and Stansted airports to Paphos on Saturday (12 February).
Services will also restart to the city on Sunday (13 February) from Newcastle and East Midlands, followed by operations recommencing from Birmingham (16 February), Glasgow (26 February), Edinburgh (16 March) and Belfast (30 March).
In addition, the companies will operate flights and holidays from Bristol airport to Paphos for the first time from 6 March.
The firms reported strong demand for flights and holidays to Cyprus, with Paphos proving to be a popular choice for holidaymakers looking to get away this February half-term, Easter and during the summer.
Flights and holidays will also restart to Larnaca from Birmingham, Bristol, East Midlands, Edinburgh, Glasgow, Leeds Bradford, Manchester, Stansted and Newcastle from 27 March.
Steve Heapy, chief executive of Jet2.com and Jet2holidays, said the companies have seen strong demand from customers looking to "make the most of being able to enjoy a much-needed getaway in the sunshine".
"We are delighted to be restarting our award-winning flights and package holidays to Paphos and seeing our flights take off once again to the stunning island of Cyprus," he added.
"We know how much holidaymakers have missed out on being able to enjoy a getaway to Cyprus with Jet2.com and Jet2holidays, so the return of these services are proving popular with customers and independent travel agents."
