The new policy will go into effect across Norwegian Cruise Line, Oceania Cruises and Regent Seven Seas Cruises from 1 August.
The pre-embarkation testing requirement will remain in place for guests currently traveling on voyages departing from destinations with local testing regulations, including but not limited to the US, Canada, Greece and Bermuda.
A spokesperson for NCLH said: "The relaxation of the testing policy is in line with the rest of the travel, leisure and hospitality industry worldwide as society continues to adapt and return to a state of normalcy.
"The company continues to strongly recommend all guests be up to date on vaccination protocols and test at their convenience prior to travel."
