The body urged the government to instruct local authorities to prioritise businesses in the sector when it comes to allocating the fund.
The alliance asked for the financial support, which was announced by chancellor Rishi Sunak in March 2021, to cover business rates since March 2020 through to March 2023.
Legislation that will enable the release of the support package is due to be completed by the House of Lords on Wednesday (8 December).
In a letter to housing secretary Michael Gove, the Tourism Alliance claimed that over the last two years the sector has suffered a decline in revenue of more than 70%.