Passengers will no longer be required to wear a mask onboard, take a pre-departure Covid-19 antigen test or fill out a health questionnaire. Testing will still be carried out on Douro itineraries.
Measures such as extended cleaning and disinfection protocols remain in place to ensure hygiene standards are upheld.
Lucia Rowe, managing director of A-Rosa River Cruises UK and Ireland, said the protocols have been relaxed following "careful consideration by our experienced team".
"We continued with some measures after the legal requirement to do so was removed by various destinations, as we felt it was important to do so at the time," she added.
