The store, which will be at the Fort Kinnaird Retail Park, is set to open later this year, creating around 16 jobs in the local area.
It is the fifth Barrhead store in the Edinburgh area and will be dedicated to long-haul travel and cruise and will also feature a foreign currency unit, the company said.
The Scottish agency said additionally investing millions of pounds in new store launches across the UK in 2018 including shop fit outs staffing, technology, training, IT and support.
Bill Munro, chairman of Barrhead Travel, said: “We know more and more customers are booking in store because they want the expertise of our specialists.
“We really look forward to welcoming both returning and new customers to our new location. Holidays are an emotional purchase and customers want to feel they are getting the best possible value for money, sound advice from genuine specialists and to know that their holidays are fully financially protected.”