The move increases the number of city break destinations the airline and operator will have on sale this winter to 12.
Birmingham will gain two new weekly services on Mondays and Fridays to the Austrian capital between 10 November 2023 and 29 April 2024, with Manchester operating the new services on Mondays and Fridays from 29 September 2023 to 20 May 2024.
The companies have also introduced new services to Prague and Krakow from East Midlands airport. Two new weekly services will depart to each destination from 9 November 2023 to 19 May 2024.
The firms have also added Prague to its Christmas markets schedule for the upcoming winter period from Edinburgh airport. Two weekly services will be available from 30 November to 17 December 2023.
The expansion comes in response to a reported uptick in demand from customers and independent agents looking to book a city break this winter.
Steve Heapy, chief executive of Jet2.com and Jet2holidays, said: "We are seeing strong demand for city breaks this winter, with more people than ever wanting to visit their favourite city break destinations.
"As always, we want to give customers and independent travel agents what they want, so have added flights and city breaks to Vienna throughout the whole of the winter season."
