The programme, which will air on Quest Red every Wednesday at 9pm from 29 September to 3 November, will see Coleen, Anne, Maureen, Linda and Denise sail the British Isles onboard the vessel during its inaugural season.
The sisters set sail from Southampton and called at Liverpool, Greenock, Portland and Belfast.
The series culminates in a final one-off performance in the ship’s 900-seat theatre with all five Nolans performing together on stage for the first time since 1978.
Antonio Paradiso, managing director of MSC Cruises UK and Ireland, said the line received a "fantastic response" from guests after the sisters cruised onboard MSC Grandiosa during the first series.
"After such a challenging year for all of us, it was amazing to welcome the Nolan sisters on board MSC Virtuosa during the restart of our beloved cruise industry this summer," he added.
"We saw such a fantastic response from our loyal MSC customers, as well as avid cruisers, who loved seeing the sisters enjoy our ship’s facilities in the first series, so it made total sense to bring the sisters back for a much-needed pick-me-up."
