The store includes interactive touch screens offering information on destinations and videos of resorts and hotels to help customers search and narrow down their holiday choices.
The shop will also offer a Travel Money desk, Welcome Bar and a self-service computer for customers to access reviews and read about excursions.
The opening comes as Tui confirms a new route to Dalaman from Liverpool John Lennon Airport for summer 2019. Tui is also offering thousands of free kids’ places across all Tui and First Choice holidays.
Tui head of retail Ste Bracegirdle said: "We’re really looking forward to the opening of our brand new TUI store in Liverpool, where we will be able to offer customers a state-of-the-art booking experience to find the perfect holiday for them.”