The three-year agreement with the Antalya-based group includes the addition of 10 luxury properties from 2024.
Andrew Flintham, managing director of Tui UK and Ireland, said: "I am delighted that we’re working with Rixos on an exclusive basis, so our customers can enjoy their luxurious resorts in popular destinations like Turkey and Croatia.
"The demand for quality, all-inclusive accommodation remains really strong, and this partnership will now provide Tui customers with outstanding choice."