Applications for the seven-night trip, which departs on 16 October, are open until 2 September. The successful applications will fly direct from Manchester onboard one of Tui's Boeing 787 Dreamliner aircraft.
Tui said the trip, for which it has partnered with Universal Orlando Resort and Visit Orlando, has been designed "to give agents first-hand experience of one of the world's most popular holiday destinations, helping them build their knowledge and confidence when selling Orlando holidays to customers".