Chief Executive Richard Hodgson called the move "a significant milestone" for the 30-year-old tour operator and added that it allows it to bring "its renowned expertise" to the high street.
"This partnership will allow us to engage new clients through immersive in-store experiences and to really bring our unique offering to life," said Hodgson.
"Crucially, this initiative complements – rather than replaces – our steadfast commitment to travel agent partners, who remain central to our growth strategy.”
The concessions will open from this November onwards.
Katie Papakonstantinou, Director of Services and Hospitality at John Lewis, said: “A key role of our stores is to deliver experiential services where human connection makes all the difference.
"Partnering with Audley Travel expands that in-store offering, bringing bespoke trip planning under the same roof as our home and lifestyle experts.”
Last month, Audley Travel told TTG was "looking at options for its first retail presence in the UK".
The concession stores will be based in Cardiff, Cribbs Causeway, Cambridge, Chelmsford, Edinburgh, Norwich, Reading, Poole, Oxford Street, Peter Jones in London, High Wycombe, and Southampton.
New retail operations lead
Tui's former Head of Retail Stephen Bracegirdle has joined Audley permanently as Head of Retail Operations following a seven-month stint as a Business Development Consultant.
He confirmed his new role on LinkedIn on Monday (7 September) soon after Audley announced it would take over the concession stores in John Lewis that Kuoni had vacated.
He posted: "I’m delighted to share that I’ve joined Audley Travel permanently as Head of Retail Operations. Having spent the past seven months working with Audley as a Business Development Consultant, helping to develop our new retail proposition and partnership with John Lewis, I’m incredibly excited to now be taking on the responsibility of leading our Retail Operations.
"For me, it’s a fantastic opportunity to combine my passion for travel, retail, people and customer experience, while helping to build something new for a business with such a strong reputation for exceptional service and expertise.
"I’ve really enjoyed getting to know Audley and its people over the last seven months, and I’m very proud to now be joining the team permanently."