MSC Cruises’ MSC Virtuosa was the first ship to welcome passengers at the terminal.
The facility is fitted with shore power, allowing vessels with the necessary technology to "plug in" once it is commissioned later this year.
Last year, Associated British Ports announced the new £55 million multi-user terminal in partnership with MSC Cruises and Norwegian Cruise Line Holdings.
Alastair Welch, director of ABP Southampton, said the "next-generation-ready" terminal would strengthen the port’s position as "Europe’s leading cruise turn-around port".
