Journey Latin America said none of its customers were near the epicentre, which was close to the coastal town of Pedernales, when the 7.8-magnitude quake struck the country’s Pacific coast on Saturday.
To date, 413 people are confirmed to have been killed during the tremor, with around 2,500 people injured.
A spokesperson for the operator said: “We have spoken to our suppliers and none of our clients currently in Ecuador have been affected by the earthquake.
"The quake struck in an area that we don’t often send clients to – the main tourist areas such as Galapagos, the Amazon and Quito are unaffected and operating as normal.
“Clients who are booked to travel will not be affected and can continue with their holidays as planned.
“We will continue to monitor the situation in case of further aftershocks but in the meantime are working with our suppliers to make sure we are up to date with any further developments.”
Meanwhile, the Ecuador’s Ministry of Tourism has issued an update on the status of its tourist locations, reporting that infrastructure including airports, hotels and ground transport for the Amazon rainforest, Andes Mountains and Galapagos Islands all remain fully operational.
In a statement, minister of tourism Fernando Alvarado said: “Our thoughts and prayers go out to the families who have lost loved ones. Ecuadorians are resilient and hardworking people and have joined forces to help their countrymen in their time of need.
“We are looking forward to collaborating with our tourism industry partners and friends across Ecuador and from around to world to help rebuild the areas most affected along Ecuador’s coast and restore tourism to the region.
"Visitors traveling to Ecuador or planning a visit to unaffected areas can feel confident that their trip will not be impacted and feel secure to continue with their plans to visit our country.”