However, in line with UK government and EU Charter guidance, the airline "strongly recommends" passengers still wear a mask throughout their flight.
Under current guidance, it is still mandatory for customers aged 12 and above travelling on Tui Airways to and from Wales and Scotland to wear a mask for the duration of their flight unless medically exempt.
Passengers may still be required to wear a mask when leaving the aircraft and inside airports.
It is also mandatory for customers aged two and above travelling to the US to wear a mask throughout their flight, and for those aged six and over flying to Italy.
Guests have been reminded to carry evidence if they are medically exempt from wearing a face covering.
The move comes after Jet2.com became the first major UK airline to relax its requirement for passengers to wear a face covering onboard its flights.