The route connecting the Scottish capital with Stansted ended at the start of October following a rethink by the airline.
Edinburgh airport chief executive Gordon Dewar said the decision to do so resulted in 48,000 fewer passengers using the airport in November, bringing the total to 1,000,246.
He added the airport had been further impacted by airlines introducing their winter schedules, which typically feature fewer flights.
Dewar said the impact of the Ryanair route loss was dissipated by strong performances elsewhere in the domestic market with British Airways growing frequencies on its Heathrow connection while Loganair launched a new route to East Midlands.
