WTTC members such as Accor, Carnival Corporation, Hilton, Internova Travel Group, Marriott International and MSC Cruises have donated rooms, transport, clothing, food, shelter, urgent supplies and money to help those impacted by the conflict.
Meanwhile, in Ukraine, hotels have stayed open to offer a base to charities, journalists, and those in need of accommodation.
Julia Simpson, WTTC president and chief executive, said there has been an "outpouring of support" from the global travel and tourism industry.
"Hotels have opened their doors to receive refugees, and in Ukraine, teams on the ground are keeping hotels open for aid agencies, journalists and those stranded and desperate," she added.
"Cruise lines and airlines have transported supplies, and across the board the response has been incredible, and I salute the courage of teams on the ground. WTTC and our members stand for peace and respecting the national sovereignty of every nation and our hearts go out to all those who are suffering from this brutal invasion."