Tui UK has confirmed it will not operate its flights and holidays departing from England and Wales from Thursday 5 November, up to and including Wednesday 2 December.
All holidays scheduled to depart between Monday 2 November and Wednesday 4 November will operate as normal.
With different restrictions in place for Scotland, package holidays departing from there are currently unaffected.
All Tui UK customers with accommodation-only bookings can also still travel if they wish, or they can choose to amend for free, the operator said.
Additionally, Marella Cruises is extending its pause in operations up to and including 16 December. The launch of the Tui River Cruise programme is also postponed, with three ships now set to launch in March and April 2021.
Tui UK will be proactively contacting all customers impacted by these changes over the coming days to discuss a range of flexible options available to them, Tui said.
These include amending for free with a "generous booking incentive", receiving an Atol-protected refund credit note with a booking incentive, or requesting a full cash refund.
Tui will now place summer 2022 holidays on-sale from tomorrow, Tuesday 3 November.
Andrew Flintham, managing director of Tui UK and Ireland, said: “The UK government’s latest decision to implement a second national lockdown is another blow for the travel industry, which has already been brought to its knees after almost eight months of continued uncertainty.
"However, public health must be the priority right now and we therefore cannot take customers on holiday from England and Wales from 5 November up until 2 December.
“We appreciate that the government has recognised the toll these restrictions will have on the sector and its employees and we’re pleased that the furlough scheme has been extended throughout November.
"However, these new measures reiterate the vital importance of a move to airport testing to allow those who want to travel to do so safely.
“We will continue to monitor the government’s advice and review our holiday programme in line with this. We hope we can take our customers away to the sunshine soon so they can escape the long, cold winter nights.”