The Professional Pilots Association (PPU) notified the airline of its intention to ballot members on Monday afternoon (November 12).
Virgin doesn’t currently recognise the PPU and instead recognises the British Airline Pilots Association, or Balpa.
The PPU though is understood to represent more than half of Virgin’s 800 pilots.
The union has long been in dispute with Virgin over recognition, and latterly, proposed changes to pilots’ pension schemes.
Despite it representing more pilots that Balpa, the PPU has been excluded from the airline’s benefits review.