The non-profit organisation raised AUS $20,000 (£11,250) to deliver meals to around 1,700 people, including cooks and porters whose livelihoods depend on international tourism.
Medlife supports low-income communities in Latin America, offering access to medicine, education and food aid.
"The entire tourism industry has been severely impacted by this pandemic," said Intrepid Travel general manager Latin America, Gary Cohen.
"As a sustainable tour operator, we are committed to giving back and supporting local communities even through the most challenging times.