The line said the move was in the best health and wellness interests of guests, crew and those living in the places where the yacht specialist plans to call.
"Many Windstar destinations and some airlines have announced, or are considering, vaccine mandates, providing further support for the new Windstar policy," said the line in a statement.
President Christopher Prelog said vaccination was "another layer of safety" for all, stressing the line’s insistence on vaccination was the "responsible course of action".