The deal should mean access to more service globally for Flybe customers via five UK airports and two European airports, Paris Charles de Gaulle and Dusseldorf.
UK cities expected to benefit from the codeshare partnership includes Aberdeen, Belfast, Southampton and Birmingham.
Vincent Hodder, Flybe chief revenue officer, said: “Our new codeshare agreement with Singapore Airlines is another exciting development for Flybe.
“It further strengthens our ability to connect our regional customers to long-haul destinations with our ‘One Stop to the World’ proposition and also serves to boost local economies by encouraging inbound business and leisure travel.”
Sheldon Hee, Singapore Airlines general manager UK and Ireland, added: “Singapore Airlines’ new partnership with Flybe greatly increases our reach throughout the UK by improving opportunities for its regional customers to access the high quality service and comfort that Singapore Airlines is known for, all from their local airport.
“We are proud to keep finding new ways for UK customers to access our flights from ever closer to home.”