B2B calls will now be handled through Cook’s two main contact centres in Peterborough and Falkirk.
Cook’s contact centre staff support its retail shops with group and scheduled bookings.
Staff had previously worked at Cook’s regional contact centres before their closures and were moved to two main bases in Peterborough and Falkirk.
Those who chose not to move worked at home.
Cook said staff were informed of the redundancies yesterday and will be offered the chance to be redeployed in shops or the contact centres.
A spokesperson confirmed that the firm’s B2C homeworking roles would not be impacted by the redundancies.
The news follows Cook’s announcement in February to close 39 stores as part of a review of its retail network.
That decision came on the back of plans to shut 28 shops in October last year, affecting in total around 400 staff.
Kathryn Darbandi, director of retail and customer experience, said: “As part of the continued transformation of our business we are making some changes to the structure of our contact centre.
“This means that some roles are at risk of redundancy, which will unfortunately affect a number of our homeworking colleagues. We are talking to everyone affected about different roles within Thomas Cook and we hope to retain as many colleagues as possible.”