Help the Santorini Donkeys urged for the rule to be put in place after a surge in the number of “obese” holidaymakers was reportedly leaving the mules with spinal injuries.
Donkeys have traditionally been used to transport people over hilly areas of the Greek island which vehicles cannot access.
Animal rights activists claim a rise in overweight visitors is forcing donkeys to carry ever-heavier loads while they already work long hours with little shelter, rest and water.
A spokesperson told MailOnline: “It’s recommended that animals should carry no more than 20% of their own body weight.