Jet2.com and Jet2holidays have suspended their Cyprus flight and holiday programmes owing to the country’s "prohibitive" entry criteria.
All trips to Larnaca and Paphos scheduled to depart up to and including 16 August have been cancelled. Trade clients will be refunded automatically.
The airline and operator has urged the Cypriot authorities to bring the country’s entry requirements in line with other destinations.
In a statement, it confirmed it was in discussion with Cypriot officials. Holidaymakers due to travel from 17 August onwards will be updated "in due course".
It comes after Jet2 was forced to first suspend its mainland Spain programme earlier this week, and then also its Balearic and Canaries programmes owing to the government’s decision to revoke Spain’s quarantine-free travel corridor and the Foreign Office’s decision to advise against all but essential travel to Spain in its entirely, including its islands.
"Because of prohibitive entry restrictions into Cyprus, we have no choice but to suspend flights and holidays to Larnaca and Paphos up to and including 16 August," said Jet2.
"We continue to urge the Cypriot authorities to review the entry criteria in line with other destinations so our customers can enjoy their well-deserved holidays.
"Where customers are affected by any programme changes, we will be automatically cancelling affected trade bookings with a full refund.
"For customers who are due to travel to Cyprus from 17 August onwards, we are continuing to liaise with the authorities in Cyprus and we will provide further updates in due course.
"We would like to thank our customers and independent travel agency partners for their understanding and patience."
Cyprus is currently shut to anyone who has been in the UK in the past 14 days, although this rule is set to be relaxed on 1 August.
However, UK holidaymakers wishing to travel to Cyprus will still have to provide a negative PCR test on arrival, obtained up to 72 hours before travel.
The test results can be presented as an email or SMS/text message, but must include the date and time the test was taken, and state the type of test (PCR).